Vietnam is undergoing a period of rapid economic development, and the demand for livestock has dramatically increased. To reduce disease risks along the supply chain, USAID and Australia’s Department of Foreign Affairs and Trade (DFAT), through the UN’s Food and Agriculture Organization, have supported Bac Giang Department of Livestock Production to develop and pilot a checklist for auditing veterinary hygiene conditions. The checklist and application guidelines were based on the Ministry of Agriculture and Rural Development’s regulation on minimum biosecurity measures for small hatcheries. Biosecurity in poultry production is a set of preventive measures such as movement control, cleaning, disinfection, and vaccination to prevent disease introduction and spread. So What? This activity helps improve the existing auditing system for hatchery certification on veterinary hygiene conditions, and contributes to disease risk management along the supply chain.